Strain PCC 7120 Heterocyst Distribution in Anabaena sp . Function of the HetN Factor Influencing Subcellular Localization

نویسندگان

  • Antonia Herrero
  • Jeff Elhai
  • Conrad W. Mullineaux
  • Enrique Flores
  • Laura Corrales-Guerrero
  • Vicente Mariscal
  • Dennis J. Nürnberg
چکیده

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CharacterizationofHetN, a protein involved in heterocyst di¡erentiation in the cyanobacteriumAnabaena sp. strainPCC7120

HetN, a putative ketoacyl reductase, is required for heterocyst pattern maintenance in the filamentous cyanobacterium Anabaena sp. strain PCC 7120. The hetN gene, when present on a multicopy plasmid, is able to suppress heterocyst differentiation. Little is known about the biochemical properties of HetN. In the present study, we found that HetN could hydrolyze ATP or GTP in vitro, and that this...

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patS minigenes inhibit heterocyst development of Anabaena sp. strain PCC 7120.

The patS gene encodes a small peptide that is required for normal heterocyst pattern formation in the cyanobacterium Anabaena sp. strain PCC 7120. PatS is proposed to control the heterocyst pattern by lateral inhibition. patS minigenes were constructed and expressed by different developmentally regulated promoters to gain further insight into PatS signaling. patS minigenes patS4 to patS8 encode...

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Epistasis analysis of four genes from Anabaena sp. strain PCC 7120 suggests a connection between PatA and PatS in heterocyst pattern formation.

The hetR, patA, hetN, and patS genes are part of a regulatory network that regulates the differentiation and patterning of heterocysts in the filamentous cyanobacterium Anabaena sp. strain PCC 7120. In this report, the epistatic interactions of mutant alleles of these four genes have been used to refine our understanding of their relationships to one another. The hetR gene was necessary for dif...

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Relationships between the ABC-Exporter HetC and Peptides that Regulate the Spatiotemporal Pattern of Heterocyst Distribution in Anabaena

In the model cyanobacterium Anabaena sp. PCC 7120, cells called heterocysts that are specialized in the fixation of atmospheric nitrogen differentiate from vegetative cells of the filament in the absence of combined nitrogen. Heterocysts follow a specific distribution pattern along the filament, and a number of regulators have been identified that influence the heterocyst pattern. PatS and HetN...

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تاریخ انتشار 2014